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Palmatine a Novel Anti‐adipogenic and Anti‐diabetic Alkaloid from an Indian Medicinal Plant Tinospora cordifolia
Author(s) -
Vasanthi Hannah Rachel,
Kannan Sangeetha Marimuthu
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.112.8
Subject(s) - palmatine , tinospora cordifolia , berberine , rosiglitazone , adipogenesis , pharmacology , traditional medicine , adiponectin , insulin resistance , chemistry , diabetes mellitus , medicine , biology , biochemistry , endocrinology , adipose tissue
Palmatine a naturally existing alkaloid isolated from a medicinal plant Tinospora cordifolia commonly used in Indian System of Medicine for various ailments has been tested for its biological activities. Palmatine in 3T3‐L1 (murine pre‐adipocytes) cells effectively inhibited adipocyte differentiation and promoted glucose consumption. Quantification of genes (Glut‐4, PPARα, PPARγ, LPL, FAS and Adiponectin) involved in diabetes and obesity using Real Time PCR and comparing the activity of Palmatine with specific inhibitors of insulin pathway (IRTK, PI3K, Glut 4) reveals that Palmatine is a novel anti‐adipogenic and anti‐diabetic molecule. It is also evidenced by the up regulation of Glut‐4 and PPARα; and down regulation of PPARγ, LPL and FAS. Oxidative stress is the common pathway linking and intensifying in all metabolic disorders. However, the anti‐oxidant property of Palmatine studied by hydrogen peroxide induced cytotoxicity study in 3T3 L1 cells was found to be mild as compared to the proven antioxidant flavonoid Quercitin. In conclusion, the results strongly support the anti‐diabetic, anti‐adipogenic and mild antioxidant activity of the naturally occurring Palmatine in Tinospora cordifolia an antidiabetic‐medicinal plant used in India which could serve as a lead pharmaceutical agent to benefit the growing diabetic/obese population. The work was sponsored by the Department of Science & Technology, Govt. of India