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Exposure to tributyltin alters the secretion of tumor necrosis factor alpha from human lymphocytes
Author(s) -
Hurt Kelsi,
Whalen Margaret M.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1119.7
Subject(s) - tumor necrosis factor alpha , secretion , cytokine , immune system , tributyltin , biology , inflammation , innate immune system , microbiology and biotechnology , immunology , endocrinology , ecology
Tributyltin (TBT) is an organotin compound that contaminates the environment and has been found in human blood samples. TBT‐exposed mammals show increased incidences of tumors. Human natural killer (NK) cells are the earliest defense against tumors and viral infections and secrete the cytokine tumor necrosis factor (TNF) alpha. TNF alpha is an important regulator of both the adaptive and innate immune responses. It promotes the inflammatory response and an association between malignant transformation and inflammation has been established. Previously, we have shown that TBT was able to interfere with the ability of NK cells to lyse tumor target cells. It important to determine if TBT can alter cytokine secretion by NK cells as this is also a critical function of these cells. The current study examines the consequences of 24 hour, 48 h and 6 day exposures to TBT on TNF alpha secretion by highly enriched human NK cells as well as mixture of T cells and NK cells. The results indicate that concentrations of TBT of 200 ‐ 2.5 nM caused significant alterations of TNF alpha secretion. Supported by NIH grant S06 GM008092‐35