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The Role of Eosinophils in Aspergillus fumigatus Lung Infection
Author(s) -
Soriani Frederico,
Russo Remo Castro,
Nogueira Camila Rodrigues Chaves,
Sousa Lirlândia Pires,
Rachid Milene,
Teixeira Mauro Martins
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1119.5
Subject(s) - aspergillus fumigatus , cxcl1 , immunology , biology , immune system , eosinophil , lung , microbiology and biotechnology , chemokine , medicine , asthma
Eosinophils are important in the pathophysiology of allergic diseases and in host immunity. Although microorganisms directly activate inflammatory cells, the mechanisms that trigger eosinophils activation and its functions in immune responses are unknown. Fungi are ubiquitous and may contribute to the development and exacerbation of pulmonary diseases. In this sense, the aim of this study was to unravel the role of eosinophils during Aspergillus fumigatus lung infection. Wild type (BALB/c) and Δdbl GATA mice were infected intranasally with 10 8 spores of an A. fumigatus wild type strain. Results show that Δdbl GATA mice had increased survival (40% versus 100% in WT mice). PMN influx in the lungs (1dpi) and airways (3 dpi) were greater in Δdbl GATA mice, but there was less protein leakage (3 dpi). Levels of eosinophils were very high in lungs and airways of WT mice after A. fumigatus infection but not detectable in Δdbl GATA mice. Airway levels of CXCL1, TNF‐α, CCL11, CCL2 and IL‐1β were reduced in Δdbl GATA mice. Interestingly, histopathological analysis did not show any differences between animals. Fungal burden was higher in Δdbl GATA mice than WT mice after 1 and 3 dpi. In conclusion, the absence of eosinophils leads to decreased production of key cytokines and increased fungal burden after infection with A. fumigatus . However, this is associated with enhanced neutrophil influx and survival, suggesting that eosinophils are crucial in setting the appropriate tone of the immune response to infection by A. fumigatus . Financial Support: CNPq, Capes, FAPEMIG.