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FORMATION OF POLYMERS INSULIN IN VITRO IN BLOOD HATCHING TIBOLONE IN MENOPAUSAL PATIENTS
Author(s) -
moral-laguna elia e del,
Valencia-Hernandez Ignacio,
Luz Linares-Perez Maria,
Rivera-Llano Aidé,
Lugo-Martinez Gabriela,
Ocharan-Hernandez Maria Esther,
Calzada-Mendoza Claudia Camelia
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1118.4
Subject(s) - malondialdehyde , tibolone , oxidative stress , insulin , endocrinology , medicine , chemistry , anthropometry , menopause
OBJECTIVE Evaluate in vitro the formation of polymers of insulin after blood incubation in menopausal patients treated with tibolone, also quantify oxidative stress markers and apply anthropometry. METHODS Were selected 18 menopausal patients 45–50 years of age, whose was treated whit tibolone for three months (2.5mg/day), whose blood was incubated in insulin, underwent same nondenaturing electrophoresis and visualize the polymers of the hormone. Additionally we evaluated markers of oxidative stress (malondialdehyde, total sulfhydryl and carbonyl groups) and anthropometric parameters. RESULTS It was observed that blood from all patients before treatment induced the formation of polymers of insulin and after treatment, only 33% remains. The oxidative stress evaluation shows reduction in damage to lipids (malondialdehyde), increased sulfhydryl groups ( they are part of the antioxidant system), but the proteins damage indicator (carbonyls) still unchanged. Regarding anthropometric assessment was observed weight reduction (p = 0.013), BMI (p = 0,035) and glucose (p =. 016). CONCLUSIONS Tibolone reduced the oxidized state of the blood and its ability to induce the formation of polymers of insulin, weight and body mass index and blood glucose.