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Genitourinary dysfunctions associated with heart failure in model of chronic volume overload in rats
Author(s) -
Claudino Mario Angelo,
Silva Fabio Henrique,
Rojas-Moscoso Julio,
Priviero Fernanda,
Antunes Edson,
De Nucci Gilberto
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1115.21
Subject(s) - medicine , carbachol , endocrinology , heart failure , stimulation , genitourinary system , volume overload , erectile dysfunction , urinary system
Chronic heart failure (CHF) patients showed that 85% have reported some episode of erectile dysfunction (ED) and 34% reported lower urinary tract symptoms (LUTS). We evaluate the relaxant‐ and contractile‐mechanisms of isolated corpus cavernosum (CC) and detrusor smooth muscle (DSM) of CHF rats (CHFR). Concentration‐responses curves to phenilephrine (PE) in CC; and carbachol (CCh), KCl in DSM were obtained from CHF rats (12wks). Nitrergic and neurogenic responses induced by electrical‐field stimulation were obtained in CC and DSM, respectively. PE contracted CC with a potency (pEC 50 ) of 5.44±0.04. The pEC 50 of PE did not change in CHFR (5.36±0.06), but, the maximal response (Emax) was increased (2.5±0.1mN/mg; P<0.05) compared with sham rats (SR; 1.9±0.1mN/mg). Nitrergic relaxation in CC was significantly decreased at higher frequencies vs SR. CCh and KCl contracted DSM with a pEC 50 of 5.42±0.07 and 0.90±0.12, respectively. The pEC 50 of CCh and KCl did not change in CHF‐R (5.68±0.07; 1.34±0.11, respect.), but, the Emax were increased (1.09±0.07; 0.88±0.05mN/mg, respect; P<0.05) compared with SR (1.45±0.10; 1.29±0.11 mN/mg, respect.). Neurogenic contractions in DSM were significantly increased in all frequencies vs SR. In conclusion, CHFR exhibit an impaired nitrergic response in CC and an increase of contractile mechanism in DSM that may contribute to genitourinary dysfunctions in CHF. FAPESP/CNPq.