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Triton X‐100 inhibits L‐type voltage‐operated calcium channels
Author(s) -
Narang Deepak,
Kerr Paul M,
Baserman Jason,
Tam Raymond,
Searle Gavin,
Manning-Fox Jocelyn,
MacDonald Patrick E,
Light Peter E,
Holt Andrew,
Plane Frances
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1115.15
Subject(s) - depolarization , chemistry , exocytosis , biophysics , electrophysiology , myocyte , vascular smooth muscle , l type calcium channel , patch clamp , voltage dependent calcium channel , inhibitory postsynaptic potential , membrane potential , endocrinology , calcium , medicine , biochemistry , secretion , biology , smooth muscle , receptor , organic chemistry
Triton X‐100 (TX‐100) is a non‐ionic detergent widely used for bioassay studies. We have used electrophysiological and functional techniques to examine the effects of TX‐100 on L‐type voltage operated calcium channel (VOCC) activity in vascular smooth muscle cells (VSMCs), cardiac myocytes and pancreatic β‐cells, cell types in which these channels play a critical functional role. TX‐100 (1.6 μM) reversibly inhibited (p<0.01) currents due to expression of recombinant L‐type VOCCs (CaV 1.2 ) reducing peak currents as compared to control. TX‐100 (1.6 μM) also reversibly inhibited (p<0.01) native L‐type VOCC currents from rat VSMCs and cardiac myocytes, and murine and human pancreatic β‐cells reducing peak currents as compared to control. In functional studies, TX‐100 (165 nM‐3.4 μM) caused concentration‐dependent relaxation of rat mesenteric arteries independent of the endothelium. TX‐100 (1.6 μM) also inhibited depolarization‐induced exocytosis in murine and human isolated pancreatic β‐cells. These data indicate that low micromolar concentrations of TX‐100 significantly inhibit L‐type VOCC activity in a number of cell types, an effect paralleled by inhibition of cell functions dependent upon activation of these channels. As this inhibitory effect occurs at concentrations below those used to solubilize proteins and lipophilic agents, it may preclude the use of TX‐100 in some bioassays.