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Inhibitory effect of Poria cocos on high glucose‐induced rat mesangial cell proliferation
Author(s) -
Yoon Jung Joo,
Lee Yun Jung,
Lee So Min,
Lee Yong Pyo,
Kim Jin Sook,
Kang Dae Gill,
Lee Ho Sub
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1103.10
Subject(s) - mesangial cell , cell growth , mapk/erk pathway , western blot , chemistry , reactive oxygen species , intracellular , microbiology and biotechnology , biochemistry , phosphorylation , biology , in vitro , gene
Mesangial cell proliferation is correlated closely with progression of renal failure. The purpose of this study was to evaluate that water extract of Poria cocos Wolf (WPC), well‐known medicinal plant, regulates rat mesangial cell proliferation under high glucose condition. [3H]‐thymidine incorporation under high glucose was significantly accelerated, which was inhibited by WPC (1 – 50 ìg/ml) in a dose‐dependent manner. Cell migration and fibronectin mRNA expression also suggest that the anti‐proliferative effect of WPC. Western blot analysis revealed that pretreatment of WPC decreased expressions of cyclins and CDKs and promoted expressions of p21waf1/cip1 and p27kip1. WPC also suppressed high glucose‐induced p38 MAPK and ERK 1/2 phosphorylation. Furthermore, WPC inhibited high glucose‐induced production of DCF‐sensitive intracellular reactive oxygen species (ROS). In conclusion, high glucose promotes mesangial cell proliferation, and that WPC has an inhibitory action through, at least in part, inhibition of cell cycle arrest and activation of anti‐oxidant property. Taken together, these results provide that P. cocos may be a potent regulator of proliferation caused by HG exposure.