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Time dependent changes in myocardial proteins of the porcine left ventricle during pressure overload
Author(s) -
Lankhuizen Inge Marlies,
McClellan Elizabeth,
Merkus Daphne,
Hekkert Maaike te Lintel,
Juhasz Peter,
Adourian Aram,
Pinto Yigal M,
Stubbs Andrew P,
Duncker Dirk J
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1098.6
Subject(s) - ventricle , pressure overload , muscle hypertrophy , heart failure , medicine , cardiology , ejection fraction , proteomics , cardiac hypertrophy , volume overload , chemistry , biochemistry , gene
The molecular mechanisms involved in the transition of Left Ventricle (LV) hypertrophy to LV failure remain largely unknown. Here, we performed proteomic analysis on LV myocardium of 29 swine (16–18kg, n=7–8/group) subjected to LV pressure overload by Aortic banding (AoB; peak systolic pressure gradient 50–70mmHg) or sham procedure. One or 3 week(s) later LV (epi‐ and endocardial) tissue was collected for iTRAQ‐proteomics followed by MALDI MS/MS. Identification of the proteins was done using InuProt KB, the NCBInr database and databases from Ensemble. AoB resulted in LV hypertrophy measured by an increased LV weight compared to sham operated animals after both 1 week (56±2g vs. 47±2g, p<0.05) and 3 weeks (84±10g vs. 54±4g, p<0.05). Ejection fraction (EF) was preserved up to 3 weeks after AoB (51±4% vs. 55±1% in sham), indicating compensated LV hypertrophy. In the proteomics study ~1000 proteins were measured in all 58 samples; ~400 of these were measured in every sample. We found 80 proteins with significantly altered expression ratios comparing AoB to sham, with mainly larger changes at the 3 weeks time point. Fewer differences were found between epi‐ and endocardial tissue. In conclusion, cardiac proteomics revealed to be a good approach to identify potential biomarkers as early predictors for LV failure.