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Estrogen supplementation in female mice yields higher sodium (Na) retention than that of testosterone in male mice
Author(s) -
Rouch Al,
Curtis Kath,
Fan Liming,
Kudo Lucia,
Toal Sheri,
Naukam Rebecca
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1096.8
Subject(s) - medicine , endocrinology , testosterone (patch) , estrogen , ovariectomized rat , chemistry , kidney , estrone , excretion , sodium , organic chemistry
Our purpose was to determine the effects of estrogen and testosterone supplementation on Na retention, blood pressure (MAP), and mRNA levels of renal Na transporters in mice. Gonadectomized female and male mice (CD1, 30–35g, 4–6 weeks old) received a pellet of 1.5 mg 17β‐estradiol (E2) and 5 mg testosterone, respectively. In metabolic cages mice consumed 1% salt diet for 10 days, 4% salt for the next 10 days, and 1% salt for the final 10 days. Na intake (Nai) and renal Na excretion (Nae) were measured daily. MAP was measured in the final 4–5 days of each period via tail‐cuff. Real‐time PCR was conducted on kidney tissue processed on the final day of each period. Nae/Nai % (mean ± se):(n=4) 1% Salt 4% Salt 1% SaltNF 57.3 ± 3.9 59.9 ± 2.5 54.0 ± 3.3 NM 47.9 ± 3.8 55.8 ± 2.9 48.4 ± 2.9 OE 28.3 ± 1.3 * 32.4 ± 1.7 * 37.8 ± 2.6 * # CT 53.5 ± 3.1 56.7 ± 3.5 47.6 ± 3.3NF & NM: normal female and male mice, OE: ovariectomized + E2, CT: castrated + T,* ‐ different from all other values (p<0.001);# ‐ different from first 1% OE period (p<0.05)MAP (mmHg) of OE mice were 95.7 ± 4.1, 79.5 ± 4.6, 93.0 ± 4.9 (1%, 4%, 1%, respectively) and not different from those of NF. PCR data showed E2‐induced upregulation of NCC, NKCC2, and ENaC mRNA. Conclusion E2 supplementation in female mice produced higher Na retention than that of T in male mice without enhancing MAP. Study supported by OCAST (OK Center for the Advancement of Science and Technology) # HR 09‐123, and OK Epscor INBRE #P20RR016478.