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Sex hormones induce gender‐related difference in renal expression of a novel prostaglandin transporter, OAT‐PG, influencing basal PGE 2 concentration
Author(s) -
Asano Shinji,
Hatano Ryo,
Onoe Kimitaka,
Matsubara Mitsunobu
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1096.2
Subject(s) - endocrinology , medicine , renal cortex , prostaglandin e , kidney , cortex (anatomy) , testosterone (patch) , basal (medicine) , hormone , prostaglandin , biology , chemistry , transporter , enzyme , biochemistry , gene , diabetes mellitus , neuroscience
A rat prostaglandin‐specific transporter (rOAT‐PG) transports PGE 2 and colocalizes with 15‐hydroxyprostaglandin dehydrogenase (15‐PGDH), a metabolic enzyme for PGs, in proximal tubules, suggesting that rOAT‐PG is involved in the PGE 2 clearance to regulate its physiological function in the renal cortex. We found that mRNA and protein expression levels of rOAT‐PG in the renal cortex were much higher in male rats than in female rats whereas there was no gender difference in the expression level of cyclooxygenase‐2, a key enzyme producing PGE 2 , and 15‐PGDH in the renal cortex. Tissue PGE 2 concentration in renal cortex was lower in male rats than in female rats, suggesting that renocortical PGE 2 concentration is primarily determined by the expression level of OAT‐PG which is differently regulated between male and female rats. Castration of male rat led to the remarkable reduction of OAT‐PG expression and significant increase of renocortical PGE 2 concentration. These alterations were recovered by the testosterone supplementation. These results altogether suggest that OAT‐PG is involved in local PGE 2 clearance in the renal cortex.