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Characterization of a neuronostatin receptor in a human kidney cell line
Author(s) -
Elrick Mollisa M,
Yosten Gina LC,
Samson Willis K
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1093.2
Subject(s) - hek 293 cells , cell culture , receptor , g protein coupled receptor , microbiology and biotechnology , embryonic stem cell , somatostatin receptor 3 , chemistry , signal transduction , biology , somatostatin receptor , biochemistry , somatostatin receptor 2 , gene , genetics
Neuronostatin (NST), a recently discovered peptide hormone derived from the somatostatin preprohormone (JBC 283:31949), was shown previously to induce c‐Fos expression in the human gastric cancer cell line (KATOIII). Preliminary data from our lab suggests that the orphan G‐protein coupled receptor, GPR107, is a cognate receptor for neuronostatin. In this study we further explored neuronostatin activity in a human embryonic kidney cell line (HEK 293). GPR107 expression was detected in untreated HEK 293 cells; we therefore hypothesized that HEK 293 cells would respond to treatment with neuronostatin with an increase in c‐Fos expression. Indeed, NST induced a concentration dependent increase in c‐Fos expression after a 60 minute exposure, as determined by real time PCR. These data suggest that HEK 293 cells are a useful model to study neuronostatin signaling. In ongoing experiments, we are attempting to compromise the production of GPR107 in HEK 293 cells using siRNA and, by assaying for responsiveness to neuronostatin, confirm findings in KATOIII cells and in vivo models that have identified GPR107 as the cognate receptor for endogenously produced neuronostatin. Supported by NIJ HL66023.