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Effect of Photo‐Irradiation on Mitochondrially‐Associated Signaling in C2C12 Muscle Cells
Author(s) -
Nguyen Linda M-D,
Larkin Kelly A,
Borsa Paul A,
Adhihetty Peter J
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1086.22
Subject(s) - mitochondrial biogenesis , myogenesis , skeletal muscle , c2c12 , ampk , bioenergetics , mitochondrion , myocyte , microbiology and biotechnology , chemistry , regeneration (biology) , phosphorylation , biology , endocrinology , protein kinase a
Laser therapy (phototherapy) is a clinical treatment used to promote tissue healing and improve muscle function in patients with musculoskeletal injury. However, the underlying cellular and molecular mechanisms responsible for the positive clinical outcomes are poorly understood. One proposed mechanism suggests specific wavelengths (710–1100‐nm) of light are preferentially absorbed by, and activate mitochondria in skeletal muscle to alter mitochondrial bioenergetics/biogenesis and potentially improve muscle regeneration post‐injury. To elucidate the underlying mechanisms, we applied varying treatment doses of phototherapy (0.42, 2.57, 8.14, 14.85, 22.80 and 29.60 J/cm2; 810–935nm; LiteCure LCT‐1000) to cultured muscle cells (C2C12 myotubes) and examined the activation of key mitochondrial biogenesis signalling proteins (AMPK, p38). Laser treatment caused phosphorylated‐AMPK (P‐AMPK) to increase in a traditional dose‐dependent manner, while phosphorylated‐p38 (P‐p38) exhibited a biphasic dose‐response. Thus, our preliminary findings indicate laser therapy evokes differential alterations in mitochondrial‐associated signaling molecules in C2C12 cells. Whether these acute phototherapy‐induced signaling changes will lead to an increase in functional mitochondria with longer‐term phototherapy in C2C12 cells has yet to be determined and is a focus of our future work.