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Angiopoietins regulate capillary network in non‐obese type 2 diabetic muscle, but VEGF is not necessary for the angiogenic response to exercise
Author(s) -
Fujino Hidemi,
Kondo Hiroyo,
Murakami Shinichiro,
Fujita Naoto,
Nagatomo Fumiko,
Ishihara Akihiko
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1086.21
Subject(s) - medicine , endocrinology , angiopoietin , soleus muscle , glut4 , ampk , vascular endothelial growth factor , diabetes mellitus , chemistry , vegf receptors , insulin resistance , skeletal muscle , biochemistry , protein kinase a , enzyme
PURPOSE To gain insight into the regulation of angiogenic process, we visualized the capillary network and evaluated the angiogenic and metabolic factors over the course of exercise training in non‐obese type 2 diabetes. METHODS The Goto‐Kakizaki (GK) rats were randomly divided into an exercise training (T2DM+Ex) and a sedentary (T2DM), while the Wistar rats were used as a control (Con). T2DM+Ex rats were trained at a low‐intensity for 60 min on a treadmill 6 times a week for 3 weeks. The 3‐D capillary network in soleus muscle was visualized using confocal laser microscopy. The expression of angiogenic factors (VEGF, KDR, Flt‐1, Ang‐1, Ang‐2, and Tie‐2), HIF‐1 alpha, AMPK and GLUT4 in the soleus muscle were determined by TaqMan probe‐based real‐time PCR. RESULTS The capillary volume of the soleus muscle was lower in T2DM than in Con rats, whereas that in T2DM+Ex was higher. Angiopoietin pathway and metabolic factors in the T2DM+Ex were significantly increased compared with those in Con and T2DM although VEGF was not changes among three groups. CONCLUSION These findings suggest that exercise training improves the regression of capillary network in diabetic muscle without the change of VEGF, and that the angiopoietin pathway in diabetic muscle could be upregulated by exercise training. Supported by Grants‐in‐Aid for Science Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology.