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Satellite cell expression of myogenic regulatory factors is altered when co‐cultured with preadipocytes
Author(s) -
Thornton Kara Jean,
Chapalamadugu Kalyan C.,
Doumit Matthew E.,
Murdoch Gordon K.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1086.20
Subject(s) - myod , myogenin , myogenic regulatory factors , myocyte , transcription factor , microbiology and biotechnology , myod protein , cellular differentiation , biology , c2c12 , chemistry , myogenesis , medicine , endocrinology , genetics , gene
Postnatal skeletal muscle hypertrophy requires activation, proliferation, and differentiation of muscle satellite cells (SCs) driven by expression of several myogenic regulatory factors (MRFs). The interaction between SCs and non‐myogenic cells within the unique microenvironment of the muscle is poorly characterized. SCs induced to differentiate in either a mono‐culture (MC) or co‐culture (CC) with preadipocytes, were isolated using laser‐assisted cell catapulting. Temporal expression of paired box transcription factor 7 ( Pax7 ) and four MRFs; myogenic differentiation factor ( MyoD ), myogenin ( Myf4 ), myogenic factor 5 and myocyte enhancement factor 2a were compared in SCs. MRF expression using TaqMan™ qRT‐PCR was performed at 0, 4, 8, 16, 24, 32, 40 and 48 hours post‐differentiation (PD). As compared to MC, differentiation of SCs in CC resulted in i) an increased Pax7 expression at 32 (p=0.05) and 48 (p=0.02) hrs PD ii) an elevated MyoD expression at 16 (p=0.05) and 48 (p=0.06), hrs PD and iii) a reduced Myf4 expression at 32 (p=0.04) and 40 (p=0.06) hrs PD. Altered MRF expression in SCs grown in CC, as compared to MC, indicates that preadipocytes can influence activation and differentiation of SCs. Furthermore, this CC is an improved model of the in vivo satellite cell niche and may aid in elucidating the complex mediation of postnatal SC activation.