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Regulation of rDNA transcription and ribosome biogenesis at the onset of skeletal muscle hypertrophy
Author(s) -
Walden Ferdinand,
Farrants Ann-Kristin Östlund,
Nader Gustavo A.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1086.13
Subject(s) - ribosome biogenesis , transcription (linguistics) , histone , epigenetics , biology , microbiology and biotechnology , chromatin , ribosomal protein , ribosomal rna , ribosome , histone h3 , muscle hypertrophy , rna , gene , genetics , endocrinology , linguistics , philosophy
We investigated the regulation of ribosomal (r)DNA gene transcription and ribosome production at the onset of skeletal muscle hypertrophy. Mice were subjected to functional overload of the plantaris muscle by bilateral removal of the synergist muscles. Mechanical loading resulted in muscle hypertrophy with an increase in rRNA content. rDNA transcription as determined by 45S pre‐RNA abundance paralleled the increase in rRNA content. Increased transcription and protein expression of c‐Myc and its downstream POL I regulon (POL1RB, TIF‐1A, PAF53, TTF1 & TAF1C) was consistent with the increase in rRNA. Other factors involved in rDNA transcription (UBF) and chromatin remodeling (WSTF) were induced by mechanical loading in a similar fashion. Chromatin‐IP revealed that these factors, together with histone H3 lysine 9 acetylation abundance relative to H3 were also enriched at the rDNA promoter. Our results demonstrate that mechanical loading induces ribosome production via epigenetic and transcriptional mechanisms at the rDNA promoter. Supported by the Swedish Research Council (VR) and the Swedish Nat Ctr for Sports Res (CIF)