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Variation of global mRNA expression in biopsies from the same human muscle
Author(s) -
Sundberg Carl Johan B,
Lindholm Malene,
Solnestam Beata Werne,
Lundberg Joakim,
Fischer Helene
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1086.11
Subject(s) - gene expression , biology , gene , skeletal muscle , myosin , phenotype , muscle biopsy , vastus lateralis muscle , messenger rna , biopsy , genetics , anatomy , pathology , microbiology and biotechnology , medicine
Human skeletal muscle biopsies obtained from the same muscle are commonly assumed to be very similar from a phenotypic and gene expression perspective, despite very few studies showing this. Therefore, our aim was to investigate differences in global gene expression pattern between and within legs of the same individual. Five sedentary subjects were included. 2 skeletal muscle samples from the vastus lateralis muscle were taken from each leg (i.e. 2 + 2 biopsies). The time between the first and last biopsy for each individual was 10 to 15 minutes. Isolated RNA was subjected to global mRNA sequencing by synthesis (RNAseq). In general, there was a very high degree of gene expression level similarity (>99 %) between biopsies within and between legs. Within the same leg, there were 7 genes with a significantly different expression for all subjects, e.g. STAT1, PRG4 (proteoglycan 4) and MYBPH (myosin binding protein H). Between legs, there were 18 differentially expressed genes. STAT1 and PRG4 were found here too, along with PDK4 and ABRA (actin‐binding Rho activating protein). Within the same leg, the number of genes with a significant differential expression varied from 119 to 4 genes. The same two individuals also had the largest and smallest variation comparing two legs. In conclusion, biopsies taken from the same individual have highly similar gene expression patterns. However, some individuals display larger variation.