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Daily Rhythm of Brain Oxidation
Author(s) -
Haushalter Kristofer,
Patel Hemal,
Ali Sameh
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1081.3
Subject(s) - reactive oxygen species , chemistry , oxidative stress , superoxide , mitochondrion , biochemistry , hydrogen peroxide , nitric oxide , antioxidant , catalase , nadph oxidase , circadian rhythm , enzyme , biophysics , microbiology and biotechnology , biology , endocrinology , organic chemistry
Circadian variations in pro‐ and antioxidants, enzymes, and markers of oxidative stress such as malonedialdehyde have been reported. However, nothing is known about the source(s) of free radicals that trigger or modulate these periodic variations. Here we explore the sources and levels of Reactive Oxygen Species (ROS) in young animal brains. We employed synaptosomal and mitochondrial preparations to study neuronal ROS and to identify contributions by mitochondria and NADPH oxidase (Nox) isoforms. We used spin trapping electron paramagnetic resonance (EPR) spectroscopy and electrochemical biosensing to detect superoxide, hydrogen peroxide, hydroxyl radicals, and nitric oxide simultaneously. We also followed daily variations of protein expression and mRNA levels of Nox subunits in mouse brain. We detected daily rhythms of substrate‐specific oxygen consumptions by mitochondria and by Nox enzymes in parallel with ROS production in the same sample. These findings suggest that circadian ROS oscillation may be involved in the interplay between metabolism and higher‐brain functions.