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Effects of estradiol on avian myogenic satellite cell proliferation and expression of heparan sulfate proteoglycans, MyoD and myogenin
Author(s) -
McFarland Douglas C.,
Velleman Sandra G.,
Pesall Jane E.,
Coy Cynthia S.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1075.20
Subject(s) - myogenin , myod , myogenesis , cell growth , myocyte , endocrinology , biology , myogenic regulatory factors , medicine , syndecan 1 , cell , microbiology and biotechnology , chemistry , biochemistry
The objective of this study was to determine the effects of estradiol on avian myogenic satellite cell proliferation and the gene expression of proteins recognized as important in regulating skeletal muscle growth and development. Estradiol (0 M 10 −5 M, 10 −6 M, 10 −9 M, or 10 −12 M) was administered in basal medium containing additional nutrients. Female‐derived pectoralis major (PM) satellite cell proliferation was stimulated at a level of 10 −9 M following 4 days of treatment. Male PM satellite cell proliferation was increased beginning at 10 −12 M estradiol. Likewise, male biceps femoris satellite cells demonstrated increased proliferation in the presence of 10 −12 M estradiol. Turkey embryonic myoblast proliferation, however, was not responsive to estradiol following 3 days administration under these conditions. Syndecan‐4 and glypican‐1 expression in male PM cells was not effected by physiological levels of estradiol (10 −9 M). However, MyoD and myogenin expression decreased in these cells when exposed to 10 −12 M and 10 −9 M estradiol, respectively. Expression of syndecan‐4, glypican‐1 and MyoD in embryonic myoblasts was not effected by estradiol. However, myogenin expression was decreased when administered 10 −12 M estradiol. The results demonstrate a direct effect of estradiol on avian satellite cell proliferation independent of syndecan‐4 and glypican‐1, and decreased expression of MyoD and myogenin coinciding with increased cellular proliferation.