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Search For RAGE In Avian Vasculature
Author(s) -
Eythrib Farid J,
Braun Eldon J
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1072.10
Subject(s) - rage (emotion) , glycation , inflammation , receptor , blot , medicine , western blot , antibody , diabetes mellitus , endocrinology , biology , immunology , chemistry , biochemistry , gene , neuroscience
Advanced glycation end‐products (AGEs) are the products of non‐enzymatic reactions between serum albumin and glucose that occur as the first stage of glycation. Receptor for advanced glycation end‐products (RAGE) is a transmembrane protein which binds AGEs. RAGE was first characterized as a member of an immunoglobulin protein family (Neeper et al. 1992). When AGE's bind to RAGE, an inflammatory response is initiated. Although this is most likely a natural immune response that results from high levels of AGEs in the blood, i. e. diabetes, the inflammation caused by activation of RAGE can cause damage to the vasculature by altering the microenvironment of the basal membrane. This creates what can be described as “leaky” vasculature (edema), which can be detrimental to patients suffering from diabetes. The mourning dove, which has a blood glucose level 4–5 times higher than that of a fasting diabetic human, does not suffer from these RAGE‐related symptoms. It was hypothesized that birds may not express the RAGE protein; however, early SDS‐Page electrophoresis and Western Blots have identified the likely presence of RAGE in mourning dove vasculature when treated with a RAGE‐specific antibody derived from rabbits. Western blot techniques will be used to more clearly identify the putative RAGE protein from avian tissue as well as immunocytochemistry will be used to localize at what level RAGE exist in the vascular tree.