Premium
The effect of temperature on the compliance of the rainbow trout heart
Author(s) -
Shiels Holly A,
Rizvi Hamid,
Fenna Andrew,
Samuel Diana
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1071.6
Subject(s) - rainbow trout , acclimatization , ventricle , trout , anatomy , stroke volume , biology , chemistry , medicine , fish <actinopterygii> , zoology , fishery , endocrinology , ecology , heart rate , blood pressure
The rainbow trout (Oncorhynchus mykiss) can vary stroke volume considerably to vary cardiac output. This requires that the chambers of the heart be compliant. Given that trout are exposed to a wide range of temperatures, it is plausible that compliance will also vary as a result of thermal acclimation. The aim of this investigation was to compare the compliance and material properties of the heart chambers (atrium, ventricle, and bulbus arteriosus) from acclimated trout (4C, 11C, and 18C). Pressure‐volume curves were generated for each chamber and compliance was determined from the gradient of these curves. Results show warm acclimation increased compliance of all chambers compared with cold acclimation. As collagen plays an integral role in tissue compliance, histology and qPCR were performed on cardiac tissue from warm and cold acclimated fish. Histological experiments established significantly higher levels of collagen in cold acclimated fish than warm acclimated fish. Furthermore, there were significantly higher levels of collagen in the compact layer than the spongy layer. In contrast there was a significant decrease in the relative abundance of collagen type I α II mRNA in cold fish suggesting multiple collagens are important in the acclimation response. This is further supported by increases in MMP2 and MMP9 (negative regulators of collagen IV) mRNA in the ventricles of warm acclimated fish.