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Tauroursodeoxycholic acid (TUDCA) improves function in cold storage hearts
Author(s) -
Porter Karen,
Mingo Tyler,
Garry Glynnis A,
Garry Mary G
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1065.6
Subject(s) - tauroursodeoxycholic acid , medicine , viaspan , taurine , ursodeoxycholic acid , cardiology , cold storage , chemistry , transplantation , apoptosis , biochemistry , amino acid , biology , unfolded protein response , horticulture
Bile acids have been used to improve various liver conditions and are now being investigated as putative therapies for neurological and cardiovascular pathologies. Tauroursodexycholic acid (TUDCA), a taurine conjugate of ursodeoxycholic acid (UDCA), has been demonstrated to reduce scar size and apoptosis resulting from myocardial infarction. Since TUDCA has been shown to protect against acute tissue injury, we hypothesized that addition of TUDCA to the cold storage solution, University of Wisconsin (UW), will improve heart function during reperfusion and lengthen the time the heart can be stored. Male rat hearts were explanted and perfused with either UW alone or UW+TUDCA (1 mM) then stored in the solution for 4 hours at 4°C. Following cold storage the hearts were reperfused with Krebs‐Henseleit buffer via a Langendorff isolated heart apparatus to measure cardiovascular function. Hearts stored in UW+TUDCA demonstrated increased left ventricular pressure (39.4 mmHg vs. 27.1 mmHg respectively) and developed pressure (90.8 vs. 64.4 mmHg respectively) compared to UW alone. Additionally, amount of time for relaxation of the heart was increased in the UW‐TUDCA group (−1648.9 mmHg/s vs. −1226.7 mmHg/s) demonstrating improvement in the heart function. These data indicate that bile acids may be beneficial in reducing tissue injury and increasing function of explanted hearts. Studies supported by AHC seed grant.