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LECTINIC BINDING OF CANDIDA GLABRATA TO OLIGOSACCHARIDES ON THE CORONARY LUMINAL MEMBRANE ALTERS FLOW‐INDUCED CARDIAC RESPONSES
Author(s) -
Torres-Tirado David,
Ramirez-Zavaleta Candy,
De Las Peñas Alejandro,
Castaño Irene,
Rubio Rafael
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1060.7
Subject(s) - stimulation , chemistry , perfusion , membrane , candida glabrata , in vitro , galactose , biochemistry , medicine , biology , endocrinology , microbiology and biotechnology , candida albicans
Candida Glabrata (CG) is a yeast that may invade humans blood stream causing dead. CG surface is lectinic and binds with high affinity to oligosaccharides ( O ) present in cell membranes, as example, coronary endothelial luminal membrane (CELM). Conversely, coronary flow (CF) stimulates auricular‐ventricular transmission (A‐V t ). CF acts on CELM lectins ( L ) and O , causing a reversible L‐O complexing necessary for CF stimulation of A‐V t . We hypothesized that CG upon binding to CELM's O prevents CF‐induced L‐O complexing; as a result inhibiting CF‐stimulation of A‐V t . In Langendorff perfused isolated guinea pig hearts A‐V t was determined at different CF. As CF (ml/min) increase A‐V t (msec) decrease. (control curve). Thereafter, to study the effects of CG bound to the CELM, CG (CGM35 sir3Δ, 3.7×10 3 cells/ml), was intracoronary given for 5 min and wash at length for 5 min and an experimental CF‐ A‐V t curve determined. The experimental curve was on top of the control curve i. e. CG upon binding to CELM increases the A‐V t value at any CF. To test the effect was due to CG bound to O , CG was displaced by perfusing sugars components of O ; mannose or Galactose. Perfusion of any of these sugars after CG they reverted its inhibitory effects on CF‐induced A‐V t stimulation. Furthermore, simultaneous perfusion of CG and any of these sugars prevented the CG‐inhibitory effects, likely competing with CELM's O for CG binding. Thus, CG upon binding to CELM's O competes with L preventing CF‐induced L‐O complexing; as a result inhibits CF‐stimulation of A‐V t .