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Modifying a high fat diet with mono‐and poly‐unsaturated fats improves coronary dysfunction
Author(s) -
Oltman Christine L.,
Dake Brian L.,
Sivitz William I.,
Yorek Mark A.,
Lamping Kathryn G.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1055.7
Subject(s) - unsaturated fat , saturated fat , sodium nitroprusside , medicine , basal (medicine) , coronary arteries , endocrinology , chemistry , insulin , food science , artery , cholesterol , nitric oxide
We tested whether partial replacement with mono‐or poly‐unsaturated fats improves coronary vascular dysfunction induced by a high saturated fat diet. We fed C57BL6 male mice high saturated fats (60% lard) for 12 weeks before substituting half of the fat with poly‐[n‐3 menhaden oil: MO or n‐6 safflower oil: SO] or mono‐unsaturated fat [olive oil: OO] for 4 weeks. After 16 weeks weight and basal glucose were elevated and glucose tolerance was reduced in lard fed compared to normal mice (4% fat). Substitution with MO but not SO or OO restored basal glucose and glucose tolerance to normal. Responses to insulin, acetylcholine (ACh) and sodium nitroprusside (SNP) were evaluated in isolated pressurized coronary arteries. Insulin–induced relaxation was attenuated in coronary arteries from mice fed a high saturated fat diet and improved with MO, OO and SO modified diets. There was a modest reduction in relaxation to ACh in mice fed high fat diet, which was not improved with any modification of diet (MO, OO or SO). SNP responses were not altered by high saturated fat diet. We conclude that short‐term enrichment of high saturated fat diet with mono‐and poly‐unsaturated fats selectively improves insulin vasoreactivity despite persistent abnormal glucose tolerance with SO and OO enriched diets. Even when glucose tolerance was restored to normal with MO, ACh‐induced relaxation was not improved in coronary arteries.