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Antioxidant, anti‐glycation and ROS scavenging activities of Plantago asiatica
Author(s) -
Nam Mi-Hyun,
Hong Chung-Oui,
Son Da-Hee,
Oh Jun-Gu,
Lee Kwang-Won
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1050.4
Subject(s) - chemistry , distilled water , gallic acid , antioxidant , quercetin , dpph , chromatography , glycation , ethanol , polyphenol , food science , flavonoid , catechin , biochemistry , nuclear chemistry , receptor
Plantago asiatica (PA) is widely distributed in Korea, China and Japan. It has traditionally been used as a folk remedy for various health benefits such as cough treatment and liver protection. The objective of this study was to demonstrate the reduction of excessive intracellular reactive oxygen species (ROS) through antioxidants and the anti‐glycation activity of PA. Dried roots of PA were boiled in ethanol for three hours, evaporated and lyophilized with a freeze‐dryer. The freeze‐dried powder of PAE was dissolved in distilled water and the solution was loaded onto a column packed with Diaion HP‐20 resin, which had been equilibrated with distilled water. The column was eluted with 2 L of 25% ethanol. Twenty‐five percent ethanol‐containing fraction (PAE‐D) was collected, and then concentrated using rotary vacuum evaporator. The concentrate was freeze‐dried. The total polyphenol content, flavonoid content, FRAP value, and DPPH‐ SC 50 of PAED were 231.7 μg gallic acid equivalents/mg dry matter (DM), 211.7 μg quercetin equivalents/mg DM, 3.23 mmol FeSO 4 /mg DM and 282.0 μg DM/mL, respectively. The effects of the PAE‐D on AGE fluorescence were dose‐dependent, reaching 71% inhibition at 1 mg/mL of extract. In addition ROS scavenging assay were performed to investigate the possibility of PAE‐D in improving endothelial dysfunction‐induced diabetic complications. Treatment of PAE‐D significantly lowered the fluorescent formation due to advanced glycation reaction. Grant Funding Source : iPET, Korea