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Hypertension related alterations in α 1 ‐adrenergic responsiveness of the rat seminal vesicle and vas deferens
Author(s) -
Yono Makoto,
Tanaka Takanori,
Tsuji Shigeki,
Irie Shin,
Sakata Yukikuni,
Otani Masayuki,
Latifpour Jamshid
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1049.1
Subject(s) - vas deferens , seminal vesicle , endocrinology , medicine , phenylephrine , testosterone (patch) , adrenergic , adrenergic receptor , chemistry , prostate , receptor , blood pressure , cancer
As hypertension may impact ejaculatory function, we investigated effects of hypertension on α 1 ‐adrenoceptors in the rat seminal vesicle and vas deferens. Spontaneous seminal emission was studied in male spontaneously hypertensive rats (SHRs) and normotensive Wistar‐Kyoto (WKY) rats. The characteristics of α 1 ‐adrenoceptor in the seminal vesicle and vas deferens of the two strains were determined using an isolated muscle bath, radioligand receptor binding and real‐time RT‐PCR techniques. Although SHRs had significantly higher serum testosterone than WKY rats, the daily mean number of ejaculatory plugs emitted and their dry weight in SHRs were significantly lower than those in WKY rats. The maximum contractile responses to phenylephrine, total α 1 ‐adrenoceptor density and expression of α 1A ‐adrenoceptor mRNA were significantly higher in the seminal vesicle and epididymal portion of vas deferens of SHRs vs WKY rats. Our data demonstrate the presence of hypertension related alterations in serum testosterone and in α 1 ‐adrenergic responsiveness of the rat seminal vesicle and vas deferens and suggest that ejaculatory function in SHRs does not mirror these hypertension related alterations. Supported by KAKENHI 21791511, Grant‐in‐Aid for Young Scientists (B) provided by the Ministry of Education, Culture, Sports, Science and Technology of Japan.