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The organosulfides diallyl sulfide, diallyl disulfide, and diallyl trisulfide accelerate benzo(a)pyrene metabolism in MCF‐10A cells
Author(s) -
Moyler Candace C.,
Nkrumah-Elie Yasmeen,
Dennis William,
Hudson Alicia,
Hammamieh Rasha,
Darling-Reed Selina
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1038.12
Subject(s) - diallyl disulfide , diallyl trisulfide , benzo(a)pyrene , chemistry , carcinogen , biotransformation , pyrene , metabolism , biochemistry , environmental chemistry , organic chemistry , apoptosis , enzyme
Benzo(a)pyrene (BaP) is a naturally and anthropogenically produced carcinogen created during the incomplete combustion of organic material. BaP is considered a dietary, occupational, and environmental carcinogen, and BaP‐associated adducts have been found in the breast tissue and milk of women. Garlic has been shown to have chemopreventive and chemotherapeutic potential, and its organosulfide compounds (OSCs) have been attributed to its anti‐carcinogenic potential. BaP is a procarcinogen, requiring biotransformation to form its highest carcinogenic potential. Normal breast epithelial (MCF‐10A) cells were treated with BaP alone; or were pretreated with either one of the the garlic OSCs diallyl sulfide, diallyl disulfide, or diallyl trisulfide at either 6, 60 or 600 ìM for 4 hrs followed by treatment with 1 ìM BaP. The media from the treatments was extracted after 12 and 24 hrs and analyzed for changes in BaP concentration using high pressure liquid chromatography. All OSCs significantly reduced the concentration of BaP in the media at 12 and 24 hrs; most likely through enhanced biotransformation by the MCF‐10A cells, which converts BaP to metabolites and intermediates. Further studies evaluating the presence of BaP metabolites in the media will determine the significance of OSC–induced BaP metabolism in MCF‐10A cells.