Premium
Proinflamatory cytokines affect dystrophin expression in cultured newborn cardiomyocytes under different stimuli
Author(s) -
Malvestio Lygia Maria Mouri,
Prado Cibele,
Campos Érica,
Celes Mara R.N.,
Ferezin Patrícia,
Silva João Santana,
Rossi Marcos A
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1036.2
Subject(s) - dystrophin , trypanosoma cruzi , vacuolization , sepsis , staining , biology , myocyte , chagas disease , microbiology and biotechnology , andrology , immunology , pathology , medicine , endocrinology , skeletal muscle , parasite hosting , world wide web , computer science
Previous studies from our laboratory demonstrated dystrophin disruption in the sepsis induced by cecal ligation and puncture in mice (CLP) and experimentally‐induced T.cruzi myocarditis. This study evaluated the dystrophin expression in cultured newborn cardiomyocytes stimulated with serum of mice infected with T.cruzi and serum of mice subjected to severe septic. Cardiomyocytes were incubated for 24 h with serum from chagasic mice or serum from septic mice, the controls cells received fetal bovine serum. The serums were obtained from C57Bl/6 mice infected with T.cruzi at the peak of parasitemia and the septic serum was collected from C57Bl/6 6 h after CLP. After incubation, the cells were processed to evaluate the expression of dystrophin, myosin and phalloidin. The immunofluorescent staining (IF) for myosin confirmed the presence of cardiomyocytes. The cells stimulated with serum from chagasic mice and septic mice presented disarrangement of myofibrils in the phalloidin staining compared to control. The IF for dystrophin showed vacuolization in the cytoplasm of cells incubated with serum from septic and T.cruzi infected mice. These results show that serum from chagasic and septic mice could be responsible for alterations in dystrophin expression in cardiomyocytes. It can be hypothesized that proinflamatory cytokines presented in serums of chagasic and septic mice could affect dystrophin expression.