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Adaptive Single Neuron Hypertensive Gene Expression Programs in the Nucleus Tractus Solitarius
Author(s) -
Brureau Anthony Yannick Bernard,
Vadigepalli Rajanikanth,
Paton Julian,
Schwaber James
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1035.19
Subject(s) - laser capture microdissection , solitary nucleus , rostral ventrolateral medulla , microdissection , medulla , neuroscience , nucleus , biology , baroreflex , blood pressure , gene expression , medulla oblongata , premovement neuronal activity , anatomy , central nervous system , endocrinology , gene , heart rate , genetics
We describe the initial adaptive response process of single neurons following elevation of the blood pressure that identify processes that may evolve over time to alter central blood pressure set point control in neurogenic hypertension. Our initial approach is to study single A2 neurons in the nucleus tractus solitarius NTS. We then study the network of NTS A2 neurons with central amygdale (CeA) and ventrolateral medulla (VLM) by injecting Retrobeads in CeA and VLM in order to identify NTS A2 neurons with projections in this network. We lift NTS A2 neurons by Laser capture Microdissection while mapping their origin in the 3‐ dimensional NTS structure to assay several hundred neurons per brain. Our 96 qPCR gene expression assays are refined from previous global gene expression studies to select a cohort that appear co‐regulated and coordinated in response to blood pressure and relevant to the A2 phenotype and to the angiotensin II AT1R receptor activated responses. Analysis of the resulting high‐dimensional dataset reveals a remarkable complexity in the response to an increase of blood pressure. These results demonstrate differential expression programs for each anatomically distinct neuronal group and neuronal type. Research Support: NIH GM 083108