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Effects of lipid emulsions on markers of inflammation in human umbilical vein endothelial cells
Author(s) -
Reyes Sarah,
Williams Janet,
Rezamand Pedram,
McGuire Mark
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1033.13
Subject(s) - umbilical vein , inflammation , postprandial , tumor necrosis factor alpha , cytokine , chemistry , biology , biochemistry , food science , immunology , endocrinology , in vitro , insulin
Atherosclerosis is a vascular inflammatory disease. Cytokines and other markers of inflammation mediate the inflammatory response. Dietary lipids are known to alter serum concentrations of markers of inflammation in vivo and specific fatty acids can alter cytokine production in vitro . Our goal was to study the effects of common dietary lipids on markers of inflammation in vascular endothelial cells. To reflect postprandial conditions, human umbilical vein endothelial cells were treated with lipid emulsions of butter, margarine, corn oil, canola oil and olive oil at concentrations of 0, 75, 150, 300 and 600 mg/dl for up to 6 h. Total RNA was extracted and quantitative PCR measured the relative abundance of mRNA of interleukin (IL)‐6, IL‐8, vascular cell adhesion molecule (VCAM)‐1, tumor necrosis factor (TNF)‐α and IL‐1β. Lipid emulsions did not stimulate expression of TNF‐α or IL‐1β. For all other genes, olive oil up‐regulated up to 2‐fold (p < 0.05) expression of mRNA compared with the other oils. The subtle changes in inflammatory markers caused by emulsions of dietary lipids are less dramatic when compared with studies evaluating specific fatty acids. Funded by United Dairymen of Idaho.