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A Plasma α‐Tocopherome in School Aged Children of Nepal
Author(s) -
Shrestha Sudeep,
West Keith P.,
Cole Robert N.,
Schulze Kerry,
Ruczinski Ingo,
Christian Parul,
Yager James,
Wu Lee,
Groopman John D.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1028.17
Subject(s) - proteome , blood proteins , selenoprotein p , vitamin d binding protein , population , medicine , chemistry , vitamin , endocrinology , biology , biochemistry , glutathione , enzyme , environmental health , glutathione peroxidase
Alpha tocopherol (α‐T) regulates numerous biological processes which may give rise to a defined plasma proteome associated with vitamin E (plasma α‐tocopherome). This cross‐sectional study among Nepalese children 6–8 yr of age explored the validity of a plasma nutriproteome that covaries with α‐T that could, in the future, be used to assess VE status. Plasma samples (n=280 to date) were obtained, processed, transported and stored by standard methods, and analyzed by HPLC for α‐T concentration (mean ± SD = 12.02 ± 3.3 μmol/L). Tandem MS iTRAQ and bioinformatics analyses were employed to identify plasma proteins and quantify their relative abundance. Of 293 unique proteins identified to date with 95% confidence, 66 proteins are correlated with α‐T [p<0.05, p (false discovery) = q<0.15; 60% at p<0.01, q<0.04). Seven of the 11 proteins most strongly correlated with α‐T are members of apolipoproteins A, B and C (all p<0.1, q<0.2). Based on functional annotation clustering (DAVID Bioinformatics Resources, v6.7) most proteins currently in the plasma α‐tocopherome are associated with secreted and signaling (enrichment score (ES), 25.9), disulfide bonding (ES 12.41), acute inflammatory and defense responses (ER 8.27), lipid and lipoprotein metabolism (ES 5.24), and complement and coagulation cascades (ES 3.05), affirming diverse biological functions of vitamin E. A diverse and clear plasma α‐tocopherome exists that, in the future, will have public health utility as a platform for population assessment of VE function and status. Funded by the Bill and Melinda Gates Foundation