Premium
Population dynamics of leukocytes in blood during early activation of the chicken immune system by E. coli
Author(s) -
Iseri Vanessa J.,
Klasing Kirk C.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1027.6
Subject(s) - cd8 , immune system , white blood cell , spleen , monocyte , medicine , endocrinology , pi , peripheral blood , population , chemistry , immunology , biochemistry , environmental health
The immune system must have appropriate levels of nutrients to support changes in leukocyte numbers during immune response, but quantitative aspects are poorly characterized. Thus, examined the early time course of peripheral blood leukocytes of adult Hyline chickens injected IV with dead E. coli . After primary injection, blood was collected and total white blood cells (WBC), CD4 + , CD8 + ), IgM + , IgG + ), and macrophage/monocyte numbers were determined. WBCs decreased at 4hr, 18hr, 24hr, and 48hr post‐injection (PI) compared to baseline (P<0.0001 for first 3, P=0.04). CD4 + decreased compared to baseline at 4hr, 18hr, 24hr, and 48hr PI (P<0.0001 for first 3, P=0.01). CD8 + decreased compared to baseline at 4hr, 18hr, 24hr, and 48hr PI (P<0.0001 for first 3, P=0.0001). IgM + decreased compared to baseline at 4hr, 18hr, 24hr, and 48hr PI (P<0.001 for first 3, P=0.004). IgG + decreased at 4hr, 18hr, 24hr, and 48hr PI compared to baseline (P<0.0001 for first 3, P=0.0009). Macrophage/monocytes decreased at 4hr, 18hr, 24hr, and 48hr PI compared to baseline (P=0.0008, P=0.003, P=0.008, P=0.01). Maximal percent decrease in cell numbers at 4hr for WBC was 96%. Decrease of cellular components in blood occurred, observed an increase in cell number in spleen, thymus and bone marrow. Converted to a whole body basis, the 70% decrease is less than the changes observed in other tissues indicating a decrease in nutrient use due to activation. Grant Funding Source : USDA Regional Research project 1013