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Dietary spray‐dried plasma influences intestinal morphology, growth performance, and organ weight of mated female mice under transport stress
Author(s) -
Song Minho,
Lee Jeongjae,
Liu Yanhong,
Soares-Almeida Juliana A.,
Che Tung M.,
Campbell Joy M.,
Polo Javier,
Crenshaw Joe D.,
Chun Ju Lan,
Seo Seongwon,
Pettigrew James E.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1027.5
Subject(s) - crypt , ileum , jejunum , duodenum , spleen , endocrinology , medicine , morphology (biology) , andrology , biology , zoology , genetics
Spray‐dried plasma (SDP) enhances gut barrier function and our previous data showed it attenuated the negative effects of inflammation caused by transport stress on pregnancy rate. We hypothesized that SDP can influence gut morphology, growth, and organ weight of mated female mice under transport stress. Mice (C57BL/6 strain) were shipped from Bar Harbor, ME to Urbana, IL on the day the vaginal plug was found [gestation day (GD) 1], arriving at the laboratory in IL on GD 3. Mice were randomly assigned to diets [0 (CON), 1, or 8% SDP]. Randomly selected mice were euthanized each day from GD 3 to 7 to collect duodenum, jejunum, ileum, and colon samples for gut morphology, and liver, spleen, kidneys, and lungs for organ weights, and to measure body growth rate. SDP increased ( P < 0.05) ratio between villus height and crypt depth of all small intestinal sections (12–38%) by increasing villus height (4–22%) and decreasing crypt depth (5–11%), and increased ( P < 0.05) crypt depth of colon (14–20%), compared with CON. SDP increased ( P < 0.05) number of goblet cells in all intestinal sections (15–35%) compared with CON. SDP increased ( P < 0.05) growth rate (21–33%) and liver weight (9–12%) compared with CON. No difference was found between 1% and 8% SDP. These data support the hypothesis that SDP influences intestinal morphology, growth, and organ weight of mated female mice under transport stress.