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Antitumor activities and immunoregulation effects of fermented sesame sauce in Sarcoma 180 tumor‐bearing mice
Author(s) -
Park Kun-Young,
Song Jiale,
Choi Jung-Ho,
Seo Jae-Hoon
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1025.20
Subject(s) - apoptosis , immune system , tumor necrosis factor alpha , in vivo , cytokine , fermentation , pharmacology , chemistry , immunology , endocrinology , medicine , biology , biochemistry , microbiology and biotechnology
The aims of this study were to investigate the antitumor activities and immunoregulation effects of fermented sesame sauce (FSeS) in vivo . Fermented soy sauce (FSS) and acid hydrolyzed soy sauce (AHSS) were used as positive controls. Female Balb/c mice were per‐orally administered with methanolic extracts of the sauces at two different dose of 12.5 mg/kg and 25 mg/kg body weight for 21 days prior to inoculation of Sarcoma 180 cells, and continued for 7 days. The tumor growth inhibition rate of FSeS group was 68.1%, and the inhibition rates were 58.1% and 9.3% in FSS and AHSS groups, respectively. RT‐PCR analysis showed FSeS could induce more apoptosis of S‐180 tumor cells through up‐regulated Bax, p21, p27, p53, caspase‐3, and down‐regulated expressions of Bcl‐2, Bcl‐xL, cyclin D1 and PCNA, than those of FSS and AHSS groups. In addition, the immunoregulation effects were determined by the levels of related immune cytokines (TNF‐α, IFN‐γ, IL‐1, IL‐2, IL‐4 and IL‐6) through ELISA assay. In cytokine assay, TNF‐α levels of FSeS (57.9 ± 2.5 pg/mL) treated group was higher than those of AHSS treated group (50.9 ± 0.4 pg/mL), but similar to the FSS group. On the other hand, FSeS can effectively increased the levels of IFN‐γ, IL‐1, IL‐2, and decreased IL‐4 and IL‐6 levels, compared to those of FSS and AHSS groups. These results suggested that FSeS can inhibit the growth of the tumor cells through up‐regulated antitumor gene expressions (Bax, p21, p27, p53, caspase‐3), down‐regulated Bcl‐2, Bcl‐xL, cyclin D1 and PCNA expressions. However, FSeS also increased the T helper (Th) cells and Cytotoxic T cells to kill the tumor cells. This research was supported by Daesang Corporation, Republic Korea.