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Anti‐adipogenic Effect of Buckwheat Sprouts treated with Methyl Jasmonate (MeJA) in 3T3‐L1 adipocytes
Author(s) -
Lee Ok-Hwan,
Yoon Bo-ra,
Lee Young-Jun,
Lim Jeong-Ho,
Kim Hyun-Jin,
Park Kee-Jai,
Kim Bum-Keun,
Jeong Jin-Woong
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1025.13
Subject(s) - methyl jasmonate , adipogenesis , nadph oxidase , chemistry , biochemistry , adipocyte , reactive oxygen species , antioxidant , rutin , quercetin , 3t3 l1 , adipose tissue , gene
Methyl jasmonate (MeJA), one of the plant stress inducers, play important roles in the regulation of plant growth and the biosynthesis of the secondary metabolites. Buckwheat sprouts contain various bioactive compounds including rutin (quercetin‐3‐ O‐rutinoside) which have a number of biological activities. We have previously shown that buckwheat sprouts treated with MeJA (TBWE) treatment significantly increased the phenolic compound amounts and the antioxidant activity. The present study is to investigate the inhibitory effects of TBWE on lipid accumulation and ROS production during adipogenesis in 3T3‐L1 cells by analyzing the mRNA expression level of adipogenic transcription factors and pro‐oxidant enzyme. 3T3‐L1 preadipocytes were treated with TBWE for up to 8 days following standard induction of differentiation. During differentiation of 3T3‐L1 preadipocytes, TBWE markedly inhibited lipid accumulation and reactive oxygen species (ROS) production compared with NBWE treated adipocyte cells. TBWE significantly inhibited the mRNA abundance of adipogenic transcription factors (C/EBPα and aP2), pro‐oxidant enzyme (NADPH oxidase, NOX4) and NADPH producing enzyme (glucose‐6‐phosphate dehydrogenase, G6PDH). These results suggest that MeJA significantly increased the amount of phenolic compound, resulting in suppression of adipogenesis and ROS production in 3T3‐L1 cells.