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Sorghum extract increases the biliary secretion of cholesterol in rats
Author(s) -
Kim Juyeon,
Noh Sang K.,
Seo Myung Chul,
Woo Guan Sik
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1025.12
Subject(s) - sorghum , triolein , cholesterol , cannula , medicine , triglyceride , endocrinology , chemistry , secretion , biology , biochemistry , agronomy , surgery , lipase , enzyme
Recently, we have shown that sorghum extract lowers significantly the lymphatic absorption of cholesterol and fats in rats. This study was conducted to examine if sorghum extract would affect the biliary secretion of cholesterol in rats. Initially, female rats were fed an AIN‐93G diet with or without sorghum extract at 0.5%. At 3 wk, each rat with bile duct cannula was infused continuously at 3.0 mL/h for 8 h via an intraduodenal catheter with a lipid emulsion containing 20.7 μmol cholesterol, 452 μmol triolein, and 3.1 μmol α‐tocopherol, and 396.0 μmol Na‐taurocholate with or without sorghum extract (control) in PBS buffer (pH, 6.4). Simultaneously, bile was collected hourly via the bile cannula for 8 h. Sorghum extract significantly increased the hourly rate of bile flow for 8 h (0.75 ± 0.05 mL/h vs. 0.91 ± 0.08 mL/h). The biliary secretion of cholesterol for 8 h was also significantly increased in rats given sorghum extract, compared with controls (3.01 ± 0.33 μmol vs. 3.73 ± 0.21 μmol). The results indicate that sorghum extract has a stimulatory effect on the biliary secretion of cholesterol in rats. The mechanism(s) by which sorghum extract enhances the biliary secretion of cholesterol remains to be investigated. Supported by the Korea Rural Development Administration (PJ007513).