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Chia seed extract does not affect zebrafish angiogenesis
Author(s) -
Meaney Mary Pat,
Nieman David C.,
Leung TinChung,
Cialdella-Kam Lynn,
Jin Fuxia
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1023.6
Subject(s) - angiogenesis , zebrafish , danio , alpha linolenic acid , biology , biochemistry , linolenic acid , alkaline phosphatase , chemistry , fatty acid , linoleic acid , polyunsaturated fatty acid , docosahexaenoic acid , cancer research , enzyme , gene
Data suggest that consumption of omega‐3 fatty acids and phenolic acids can protect against cancer. Chia seeds, which contain high concentrations of alpha‐linolenic acid (ALA), an omega‐3 fatty acid, and protocatechuic acid (PCA), a phenolic acid, have a reported protective effect against cancer. However, whether chia seed extract inhibits tumor angiogenesis is presently unclear. The purpose of this study was to determine whether treatment with select fractions of milled chia seed extract (Mila ®, LifeMax: Orlando, FL) affects angiogenesis in zebrafish embryos. TG(flk1:GFP) transgenic zebrafish ( Danio rerio ) embryos were incubated in solutions containing an ALA‐rich fraction of chia seed extract, a PCA‐rich fraction of chia seed extract, pure ALA, or pure PCA. Alkaline phosphatase staining was used to determine the effects of these treatments on angiogenesis. None of the experimental treatments affected angiogenesis in zebrafish embryos. While fractions of chia seed extract used in this study did not prevent tumor angiogenesis, they may protect against cancer by some other mechanism. Funded by: LifeMax ® Inc. and Chia Farms, Inc.