Premium
Effect of Dietary Genistein on Hormone‐dependent Rat Mammary Carcinoma Induced by Ethyl Methanesulphonate (EMS)
Author(s) -
Ono Misaki,
Koga Takaomi,
Ueo Hiroaki,
Nakano Shuji
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1023.14
Subject(s) - genistein , daidzein , equol , glycitein , isoflavones , hormone , phytoestrogens , endocrinology , medicine , chemistry , estrogen , carcinogen , biochemistry
Although genistein (GEN), a major soy isoflavone, has been shown to reduce the risk of breast cancer (BC) incidence, many experimental studies have yielded the inconsistent results. We investigated the effects of GEN on the development of BC using the EMS chemically induced rat model of hormone‐dependent BC. Female WKA rats were orally given EMS for 12 weeks and fed the soy‐isoflavone‐free NIH‐07PLD diets with or without GEN. All EMS‐treated rats fed either diet developed ER‐ and/or PgR‐positive hormone‐dependent mammary carcinoma by 24 weeks. Compared with GEN‐free diet, the addition of either low or high GEN to GEN‐free diet, which produced plasma concentrations of GEN comparable with those observed in humans consuming high or more soy diets did not show any preventive activity on the development of EMS‐induced hormone‐dependent BC. Soy‐containing food CE2, which resulted in the modest plasma concentration of isoflavones such as GEN, daidzein, equol, and glycitein, increased the latency periods, compared to either isoflavone‐free diets with (p=0.008) or without GEN (p=0.052). The body weight, total EMS uptakes, and urine estradiol concentrations were not significantly different between these 4 groups. These data indicate that GEN does not exert clear preventive effects on estrogen‐dependent mammary carcinogenesis and that isoflavones other than GEN may be responsible for the prevention of BC.