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Quercetin decreases tumorigenesis in a high fat diet‐enhanced mouse model of breast cancer
Author(s) -
Steiner Jennifer,
Davis J Mark,
McClellan Jamie,
Green Jeffrey,
Murphy E Angela
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1023.13
Subject(s) - medicine , carcinogenesis , endocrinology , breast cancer , cancer , tumor necrosis factor alpha
Consumption of a high fat diet (HFD) increases the risk for breast cancer (BrCa). Bioactive food components such as quercetin (Quer) may target processes relevant to HFD‐enhanced BrCa. OBJECTIVE To determine the efficacy of Quer in prevention of HFD‐enhanced tumorigenesis in the C3(1)SV40Tag mouse model of BrCa. METHODS Female C3(1)SV40Tag mice were assigned to a dietary treatment group (n=14–15) at 4wks of age: control (CON) diet (AIN76A; 11% kcal fat), HFD (41% kcal fat), or HFD supplemented with 0.02% Quer (QHFD). Body weight (BW) and food intake were measured weekly and palpable tumors bi‐weekly. At 18wks of age mice were sacrificed and all visible tumors were counted, and measured. Tumors were analyzed for mRNA expression of pro‐tumoral factors (VEGF, IL‐6, CD206 and TNF‐á) using RT‐PCR. RESULTS HFD intake (HFD and QHFD) significantly increased BW (wks 8–18) (P<0.05), despite no differences in kcal intake. Tumor number and volume were increased following HFD at 18wks and Quer offset this effect (P<0.05). In fact, QHFD mice had significantly lower tumor volume than CON at 18 wks. Tumor expression of VEGF, IL‐6, CD206 and TNF‐á all showed strong trends of an elevation in HFD compared to CON and QHFD. CONCLUSIONS These data provide strong support for a beneficial effect of Quer in reducing HFD‐enhanced BrCa that may be mediated by its ability to decrease pro‐tumoral factors within the tumor. Grant Funding Source : None