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Association between the plasma proteome and serum ascorbic acid concentrations in humans
Author(s) -
Da Costa Laura Anne,
García-Bailo Bibiana,
Borchers Christoph H,
Badawi Alaa,
El-Sohemy Ahmed
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1017.9
Subject(s) - ascorbic acid , vitamin , vitamin c , albumin , vitamin d binding protein , population , medicine , ceruloplasmin , chemistry , endocrinology , vitamin d and neurology , physiology , biochemistry , food science , environmental health
Vitamin C has been associated with a reduced risk of chronic diseases, but the biological pathways regulated by vitamin C are not all known. The objective was to use a proteomics approach to identify plasma proteins associated with circulating levels of ascorbic acid. Men and women (n=1,022) 20–29 years of age from the Toronto Nutrigenomics and Health Study completed a general health and lifestyle questionnaire and 196‐item food frequency questionnaire and provided a fasting blood sample. Circulating ascorbic acid was analyzed by HPLC and a mass spectrometry‐based multiple reaction monitoring method measured 54 proteins abundant in plasma. Mean protein concentrations were compared across tertiles of serum ascorbic acid using analysis of covariance. Levels of complement C9, ceruloplasmin, alpha‐1‐anti‐trypsin, angiotensinogen, complement C3, vitamin D binding protein, and plasminogen were inversely associated with levels of ascorbic acid (P<0.0009). The inverse association between ascorbic acid and vitamin D binding protein was highest in those with higher levels of total 25‐hydroxyvitamin D (interaction P=0.2). In conclusion, several plasma proteins from various physiological pathways are significantly associated with circulating levels of ascorbic acid in a population of young adults. This research was funded by the Advanced Foods and Materials Network.