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Effects of high‐fat high‐fructose diet and EPA supplementation on adipose tissue gene expression in adult rats
Author(s) -
Leray Véronique,
Jonchère Camille,
Zaman Muhammad-Quaid,
Nguyen Patrick
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1016.15
Subject(s) - endocrinology , medicine , adipose tissue , fructose , leptin , dyslipidemia , perilipin , biology , lipid metabolism , insulin , chemistry , obesity , biochemistry , adipocyte
The study of expression of genes involved in obesity‐related disorders provides a tool to elucidate the links between obesity, loss of insulin sensitivity (IS), and dyslipidemia. Our aim was to measure the effects of a high‐fructose diet and EPA supplementation on metabolic status and mRNA expression of genes involved in insulin‐signaling pathway, and glucido‐lipid metabolism in rats. Rats were given for 10 wk either control diet or high‐fat high‐fructose diet (40% fructose, 40% lipid cal), supplemented or not with EPA (120 mg/d). At the end of the 10‐wk period, body composition and IS were assessed, lipid plasma were assayed, and mRNA expression of insulin receptor, IRS1, perilipin, leptin, ACC, FAS, HSL, LPL, GLUT, TNFα, SREBP‐1c, PPARγ was semi‐quantified from adipose tissue biopsies. In rats fed with high‐fat high‐fructose diet, plasma cholesterol level was higher than in control rats, as well as ACC and FAS mRNA levels. In rats fed with the same diet plus EPA, plasma cholesterol level was lower and PPARγ mRNA level higher compared with the rats fed with high‐fat high‐fructose diet. Our results show changes in expression of lipogenic genes in rats fed high‐fat high‐fructose diet, that could reflect the obesogenic effect of such a diet. They also show that EPA could stimulate the transcription of PPARγ that could account for its beneficial effect on lipidemia.