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Elongase of long chain fatty acids family 6 (ELOVL6) and stearoyl‐CoA desaturase ‐1 (SCD1) indices suggests potential metabolic benefits in patients with a range of liver fat contents
Author(s) -
Lee Joseph J,
Bradford Dora L,
Valdez Maressa J,
Hovhannisyan Yelena,
Lambert Jennifer E,
Ramos-Roman Maria A,
Parks Elizabeth J
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1014.7
Subject(s) - chemistry , polyunsaturated fatty acid , medicine , endocrinology , postprandial , fatty acid , meal , triglyceride , metabolism , insulin , food science , biochemistry , biology , cholesterol
SCD1 and ELOVL6 enzyme activities and dietary fatty acid composition contribute to the balance of monounsaturated (MUFA), polyunsaturated (PUFA), and saturated fatty acids (SFA) in the plasma free fatty acid (FFA) pool. Given the potential lipotoxic effects of SFA, the present study investigated FFA composition in fasted and meal‐fed (42g fat, 100g carbohydrate) nondiabetic, African American and Hispanic adults (n = 14, BMI 35.6 ± 6.8 kg/m 2 , 47.7 ± 8.5 yrs) with low (< 5.6%) or high (> 5.6%) liver fat. FFA composition (mol %) was determined by GC, substrate oxidation by indirect calorimetry, and substrate metabolism using stable isotopes. Low ELOVL6 index (18:0/16:0) was associated with high postprandial insulin (r = − 0.622, P = 0.02) and lower in those with high liver fat (P = 0.01). Fasting FFA turnover correlated with FFA 18:1n9 (r = +0.694, P = 0.01) and 18:2n6 (r = +0.642, P = 0.01), and negatively with 18:0 (r = −0.731, P < 0.01) and 16:0 (r = −0.731, P < 0.01). Whole body fat oxidation correlated with 16:1n7 (r = +0.646, P = 0.02) and indices of SCD1 including 18:1n9/18:0 ratio (r = +0.558, P < 0.05) and 16:1n7/16:0 (r = +0.633, P = 0.02). Fat oxidation correlated inversely with 18:0 (r = −0.592, P = 0.03). Thus, 18:1n9 may be a biomarker for both greater FFA turnover and disposal. These data support beneficial effects of MUFA over SFA in overweight subjects and a positive influence of ELOVL6 and SCD1 on insulin sensitivity and liver fat.