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Rab2 plays a role in lipid metabolism in the intestine
Author(s) -
Chung Bomee,
Schürmann Annette
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1014.11
Subject(s) - endoplasmic reticulum , microsomal triglyceride transfer protein , secretion , golgi apparatus , transfection , lipid metabolism , chylomicron , small intestine , triglyceride , chemistry , medicine , endocrinology , microbiology and biotechnology , lipoprotein , cholesterol , biology , biochemistry , very low density lipoprotein , gene
Intestinal lipid metabolism is comprised of multi‐steps of vesicle trafficking. Rab2 is localized in pre‐Golgi intermediates and required for vesicle trafficking between the endoplasmic reticulum and the Golgi. The aim of the study was to investigate the role of Rab2 in the secretion of chylomicron in the intestine. Rab2 expression was suppressed by transfection of specific siRNA in Caco‐2 cells, an intestinal in vitro model, and lipid absorption and lipoprotein production were examined. Rab2 expression was efficiently depleted by siRNA transfection for 14 days without interrupting cell differentiation. Apolipoprotein (Apo) B100 secretion was significantly lower in Rab2 siRNA transfected cells in the basal condition, but it recovered when the cells were oil‐loaded. In the same way, triglyceride secretion was slightly decreased in the basal condition, but recovered by oil‐load. Expression of microsomal triglyceride transport protein (MTTP) was not affected by Rab2 disruption. Rab6 expression was significantly increased in Rab2 siRNA transfected cells. These data suggest that Rab2 and Rab6 are involved in chylomicron secretion in Caco‐2 cells, and compensate its role each other. Grant Funding Source : Alexander von Humboldt Foundation