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The effects of combination treatment of parenteral arginine and a nitric oxide synthase inhibitor L‐NAME on oxidative stress in rats with sub‐acute peritonitis
Author(s) -
Lo Hui-Chen,
Lee Chien-Hsing
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1013.28
Subject(s) - oxidative stress , lipid peroxidation , antioxidant , superoxide dismutase , glutathione peroxidase , nitric oxide synthase , nitric oxide , peritonitis , chemistry , tbars , medicine , pharmacology , vitamin c , arginine , glutathione , endocrinology , biochemistry , enzyme , amino acid
Using a modified rat model with cecal puncture and sub‐acute inflammation, we investigated the combination effects of arginine and L‐NAME, a nitric oxide synthase inhibitor on oxidative stress and antioxidant enzymes in the circulation and peritonitis‐affected target organs. Male Wistar rats with sub‐acute peritonitis were infused with a conventional parenteral nutrition solution or that supplemented with arginine, L‐NAME or both. Parenteral fed rats without peritonitis were used as controls. After 7 days, peritonitis‐induced alterations in TBARS, the lipid peroxidation products, in the erythrocytes and kidneys were significantly reversed by parenteral arginine. In addition, the increased activities of superoxide dismutase (SOD) and catalase in the liver were significantly alleviated by arginine supplementation. L‐NAME significantly decreased the activity of glutathione peroxidase in the liver and significantly increased the levels of vitamin C in the plasma and the activity of SOD in the kidneys. Combination treatment significantly increased plasma vitamin C and pulmonary thiol group and glutathione levels. In conclusion, our results showed that parenteral arginine and L‐NAME may modify antioxidant enzymes and non‐enzyme substrates, respectively. In addition, combination treatment allows the peritonitic rats to maintain an oxidant‐antioxidant homeostasis with non‐significant alterations in lipid peroxidation and antioxidant enzyme activities.