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Fasting and refeeding regulate in different manner SNAT 2, SREBP‐1 and 2, mTOR, serum insulin in animals fed different types of protein
Author(s) -
Ortiz-Huidrobo Rosa Isela,
Marquez-Mota Claudia,
Quiñonez-Peña Alejandra,
Tovar-Lopez Eduardo,
Galvez-Mariscal Amanda,
Tovar Armando R,
Torres Nimbe
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1013.18
Subject(s) - medicine , endocrinology , insulin , sterol regulatory element binding protein , pi3k/akt/mtor pathway , amino acid , chemistry , hormone , leucine , biology , biochemistry , apoptosis , sterol , cholesterol
Several transcriptional factors or receptors have been described as sensors of carbohydrates or fatty acids to regulate the utiization of these nutrients, however there is scarce information about the sensors for amino acids. Thus, the purpose of the present work was to assess whether different types of proteins regulate serum insulin, mTOR, SNAT2, SREBP1 and SREBP2 during fasting and refeeding. Male Sprague Dawley rats were fed 20% protein diets: casein (C) soy (SY), corn gluten (G), sesame (SE), green pea (GP), black bean (BB), black bean plus corn gluten and spirulina (S) for 21 days. Rats fed with G, SE or S gained less weight that the rest of the groups. mTOR mRNA expression correlated with the amount of leucine in the diet (P<0.01). Serum Insulin, and SREBP‐ 1 and FAS showed the lowest expresion during fasting and the highest expression after 1h refeeding. SNAT 2 was repressed after refeeding and was stimulated mainly by soy and GP, and SREBP‐2 decreased in the refeeding. Thus, fasting, refeeding and protein quality regulate in different extent these genes. Supported by IcyT DF (AG).