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The Spliceosome is a Dynamic RNP Machine
Author(s) -
Guthrie Christine
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.100.1
Subject(s) - spliceosome , chromatin , rna splicing , molecular machine , microbiology and biotechnology , computational biology , budding yeast , rna , biology , rna processing , genetics , yeast , saccharomyces cerevisiae , dna , gene
Our long‐term goal is to understand at a molecular level the mechanisms responsible for the fidelity and specificity of mRNA processing, with a particular focus on the ATP‐dependent steps. We previously established that the spliceosome is a highly dynamic RNA‐protein machine that relies on RNA‐dependent ATPases of the so‐called DEAD‐box family to promote multiple conformational rearrangements. Recent advances showing that mRNA processing in vivo is largely, if not exclusively co‐transcriptional, have now broadened our perspective. Evidence will be presented that implicates important new parameters from our studies in budding and fission yeast, including the structure and modification states of chromatin, as well as the elongation rate of RNA polymerase II.