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Expression Profile of GT1‐7 Hypothalamic Cell Line Under Exposure Class I Androgen Testosterone Propionate
Author(s) -
Agosto Ibis Milady,
Vizcarrondo Giovann,
Cruz Raphael,
Brito Paul,
Garcia Liz,
BarretoEstrada Jennifer,
RoigLopez Jose L.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb96
Subject(s) - testosterone propionate , transcriptome , androgen , microarray analysis techniques , anabolism , microarray , gene expression , testosterone (patch) , gene , biology , medicine , downregulation and upregulation , endocrinology , hormone , genetics
Anabolic Androgens Steroids are synthetic chemicals derivates from testosterone that are currently divided in three major classes. Our research interest is to analyze in vitro expression profile of different classes of androgen analogs using GT1‐7 hypothalamic cell line that will guide us to understand the different responses between anabolic androgens in the Hypothalamic‐Pituitary‐Gonads circuitry. Initially we compared basal profile expression of Testosterone Propionate (TP) and its control vehicle using microarrays. The transcriptome analysis demonstrated data of 1,219 upregulated and 157 as downregulated genes. In order to confirm these results RT‐PCR analysis was used. The genes were divided in three categories up‐regulated know, up‐regulated unknown and down regulated genes. The PCR expression pattern of 10/12 unknown, 7/9 known and 3 out of the down‐regulated genes were confirmed through PCR and gel electrophoresis. This may suggest that the TP may differentially regulate the expression of these unknown genes. These report represent the first functional information about the nature of the unknown genes in which there is no molecular, biological and structural information. Later on, microarray data with other comparisons will be analyzed. This work was partially supported by the UNE URGREAT‐MBRS‐RISE grant 2R25GM066250‐05A1 and by grant P20 RR016470 from the National Center for Research Resources (NCRR), PR‐ABREE, IDEA/BRIN program a component of the NIH.