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The Effect of Acute High‐intensity Resistance Exercise and Dietary Cholesterol on PPARδ Protein Content in Skeletal Muscle
Author(s) -
Chen Vincent C.W.,
Lee Chang Woock,
Lee Teak V,
Bui Steve,
Riechman Steven E
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb594
Subject(s) - medicine , endocrinology , skeletal muscle , cholesterol , peroxisome proliferator activated receptor , resistance training , intensity (physics) , receptor , physics , quantum mechanics
PPARδ expression in skeletal muscle has been shown to increase in response to endurance exercise training. However, the effect of acute high‐intensity resistance exercise (RE) and/or dietary cholesterol (CL) intake on PPARδ expression has not been investigated in humans and is thus the purpose of this study. Fourteen untrained, healthy young men and women were randomly assigned to a high‐cholesterol (HC: 14mg/kg lean/day, n=8) or low‐cholesterol intake group (LC: 3.5mg/kg lean/day, n=6), and instructed to perform 2 bouts of eccentric RE consisting of unilateral leg presses and extensions to fatigue. Muscle biopsies were performed on both exercise (EX) and non‐exercise (NoEX) legs 22 hours after exercise. PPARδ levels were analyzed via Western Blotting. The results showed no significant difference in PPARδ levels between HC and LC groups (p=0.868) or EX and NoEX legs (p=0.325). However, there was a significant interaction between RE and CL intake in PPARδ levels (p=0.047). In HC group, PPARδ levels in EX legs (1.81±0.44 AU) were higher than NoEX legs (1.04±0.15 AU) by 74% (p=0.029) while exercise had no effect on PPARδ levels in LC group (EX: 1.36±0.31 AU, NoEX: 1.64±0.44 AU, p=0.445). Our data suggest that when combined with high CL intake, acute high‐intensity RE may enhance PPARδ expression while acute high‐intensity RE or dietary CL alone has no effect on PPARδ levels.