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Fiber‐specific expression of alpha‐actinin‐3 protein in rat diaphragm
Author(s) -
IchinosekiSekine Noriko,
Yoshihara Toshinori,
Ogura Yuji,
Sugiura Takao,
Powers Scott K,
Naito Hisashi
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb588
Subject(s) - alpha (finance) , fiber type , fiber , diaphragm muscle , diaphragm (acoustics) , skeletal muscle , chemistry , microbiology and biotechnology , medicine , anatomy , endocrinology , biology , physics , weakness , surgery , construct validity , organic chemistry , acoustics , loudspeaker , patient satisfaction
Alpha‐actinin‐3 is a major structural component of the Z‐lines in skeletal muscle that maintain the spatial relationship between myofilaments. However, fiber‐specific expression of alpha‐actinin‐3 vary among the species and different muscles. The purpose of this study was to examine the alpha‐actinin‐3 protein expression in rat diaphragm. Adult Wistar male rats (n=8) were anesthetized and the diaphragm were collected. Immunohistochemical detection of muscle fiber type (Type I, Type IIa and Type IId/x) and alpha‐actinin‐3 expression were performed. Percentage of cross‐sectional area (CSA) occupied by each fiber type were; Type I: 20.5%, Type IIa: 17.8%, and Type IId/x: 61.7%. Fiber CSAs were; Type I: 857.1 ± 58.9 μm 2 , Type IIa: 971.3 ± 41.9 μm 2 , and Type IId/x: 3529.0 ± 171.8 μm 2 . Alpha‐actinin‐3 was not expressed in Type I fiber same as previous reports in mouse and human. On the other hand, all of type IId/x fiber expressed alpha‐actinin‐3. We also observed expression of alpha‐actinin‐3 in 13.9 ± 8.9% of type IIa fibers. These observations indicated that expression pattern of alpha‐actinin‐3 in rat diaphragm was different from those of other species.