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A serum‐free chemically defined medium for the expansion of adult normal human dermal fibroblasts
Author(s) -
D'Souza Theresa,
Roderick Karen,
Walde Amy,
Bossie Alexis
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb520
Subject(s) - fibroblast , in vitro , dermal fibroblast , chemically defined medium , basic fibroblast growth factor , chemistry , wound healing , growth factor , microbiology and biotechnology , immunology , pathology , medicine , biology , biochemistry , receptor
Cultured dermal fibroblasts are used clinically for acute and chronic wound repair and as a source of collagen for cosmetic surgery. Typically cells are cultured in medium containing serum. The unknown components and variable nature of serum is a challenge in basic research and clinical applications. Alternatives to serum to supplement cell culture media is much sought after. We have developed a serum‐free chemically defined, fibroblast growth medium (TheraPEAK TM FGM‐CD TM ), to propagate primary adult normal human dermal fibroblasts (NHDF). NHDFs from several donors grown in FGM‐CD TM , proliferated for multiple passages, maintained a fibroblast phenotype and expressed fibroblast‐specific markers comparable to serum‐containing control medium. NHDFs grown in FGM‐CD TM on several different plastic surfaces maintained equivalent monolayer growth, viability and fibroblast markers. The migration capacity of the fibroblasts in FGM‐CD TM was similar to migration in the control medium. Immunofluorescence staining showed that NHDFs synthesize more collagen I in FGM‐CD TM than in serum‐containing medium. Replacement of serum‐containing medium with TheraPEAK TM FGM‐CD TM for the in vitro expansion of fibroblasts provides a more defined environment for pharmacological manipulations for research studies and for clinical applications using fibroblasts. Source of research support: Lonza BioScience