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MicroRNA has‐let‐7g inhibits proliferation, adipogenic differentiation and inflammation in C3H10T½ cells
Author(s) -
Yan Xu,
Zhu MeiJun,
Du Min
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb493
Subject(s) - adipogenesis , microrna , ccaat enhancer binding proteins , inflammation , microbiology and biotechnology , biology , transfection , adipose tissue , mesenchymal stem cell , chemistry , cell culture , transcription factor , endocrinology , immunology , gene , biochemistry , nuclear protein , genetics
MicroRNAs regulates gene regulation. We previously observed that let‐7g expression was reduced in the skeletal muscle with enhanced adipogenesis and inflammation, and hypothesized that let‐7g, a conserved microRNA, plays an important role in adipogenesis and associated inflammation. C3H10T½ mesenchymal stem cells stably over‐expressing let‐7g or a scrambled microRNA were selected following lentiviral transfection. The expression of let‐7g was 8 times higher in let‐7g infected cells compared with control cells. Let‐7g cells proliferated at 54.3 ± 10.9% of the rate of control cells. Let‐7g inhibited the mRNA expression of peroxisome proliferator‐activated receptor (PPAR)γ and CCAAT/enhancer binding protein (C/EBP)α, both are markers of adipogenesis, as well as the protein contents of PPARγ (57.1 ± 12.7% lower) and C/EBPα (56.1 ± 15.2% lower). Less adipocytes were formed in let‐7g cells. Let‐7g over‐expression inhibited the expression of inflammatory cytokines, tumor necrosis factor (TNF)α and interleukin (IL)6, demonstrating the anti‐inflammatory effect of let‐7g. In summary, let‐7g inhibits C3H10T½ cell proliferation and suppresses adipogenic differentiation. Let‐7g over‐expression also down‐regulates the expression of inflammatory cytokines. MicroRNA let‐7g may be an important target for the treatments of obesity and other metabolic diseases.